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[Effect of surface-active agents on staphylococcal coagulase]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|June 1, 1977
Summary
Certain surfactants like catamine and sulfonol inhibit fibrin formation by affecting coagulase activity. Other surfactants, such as sodium alkylsulfates, also impede this crucial blood clotting reaction.
Area of Science:
- Biochemistry
- Microbiology
- Surface Chemistry
Background:
- Fibrin formation is a critical step in blood coagulation, essential for hemostasis.
- Staphylococcus species produce coagulase, an enzyme that plays a key role in initiating the coagulation cascade.
- Surfactants are compounds that lower surface tension, and their interaction with biological processes is of significant interest.
Purpose of the Study:
- To investigate the impact of various surfactants (catamine AB, sulfonol NP-3, sodium alkylsulfates, syntanol DT-7) on coagulase-mediated fibrin formation.
- To elucidate the mechanisms by which these surfactants affect the kinetics and components of the fibrin formation reaction.
- To assess the influence of these surfactants on the coagulation of human plasma by pathogenic Staphylococcus.
Main Methods:
- Studied the reaction kinetics of fibrin formation using partially purified coagulase in the presence of different surfactants.
- Determined the effects of varying concentrations of catamine AB, sulfonol NP-3, sodium alkylsulfates (C10-C16), and syntanol DT-7.
- Analyzed the impact on enzyme activity, intermediate reaction products, and the coagulation of human citrate plasma.
Main Results:
- Catamine and sulfonol significantly inhibited fibrin formation velocity at concentrations above 0.002% and 0.01%, respectively.
- Catamine primarily acted by inactivating the coagulase enzyme, while sulfonol affected intermediate reaction products.
- Sodium alkylsulfate inhibition increased with alkyl chain length, with tetradecyl sulfate sodium showing the greatest effect; syntanol did not inhibit enzyme activity but increased coagulase production in Staphylococcus cultures.
Conclusions:
- Specific surfactants, notably catamine and sulfonol, can effectively inhibit the coagulase-driven fibrin formation process.
- The mechanism of inhibition varies among surfactants, involving direct enzyme inactivation or interference with reaction intermediates.
- Catamine, sulfonol, and to a lesser extent syntanol, can retard the overall coagulation of human plasma mediated by pathogenic Staphylococcus.