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Tissue water content in rats measured by desiccation
R F Reinoso1, B A Telfer, M Rowland
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, England, UK.
Journal of Pharmacological and Toxicological Methods
|December 24, 1997
Summary
This study determined rat tissue water content, finding testes had the highest and adipose the lowest. These findings are crucial for physiologically based pharmacokinetic models.
Area of Science:
- Physiology
- Pharmacokinetics
- Comparative Anatomy
Background:
- Accurate tissue water content is essential for physiologically based pharmacokinetic (PBPK) models.
- Previous studies have provided data for some tissues, but a comprehensive dataset for all PBPK-relevant tissues is lacking.
Purpose of the Study:
- To quantify water content across 14 different rat tissues.
- To assess the influence of body weight and tissue location on water content.
- To provide a complete dataset for PBPK model development.
Main Methods:
- Tissue water content was measured using desiccation to constant weight at 40-50°C.
- Tissues were analyzed from two groups of rats with differing body weights (200-250g and 270-430g).
- Water content was corrected for residual blood volume in specific tissues.
Main Results:
- Testes exhibited the highest water content (0.861 ml/g), while adipose tissue had the lowest (0.183 ml/g).
- Significant differences between uncorrected and corrected water content were noted for spleen, lungs, kidneys, heart, liver, and brain.
- Tissue water content was independent of rat body weight and consistent between paired organs (kidneys, testes) and bone.
Conclusions:
- This study provides a comprehensive dataset of rat tissue water content.
- The data supports the development and refinement of physiologically based pharmacokinetic models.
- Water content variations across tissues and the impact of residual blood are important considerations for modeling.