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Updated: Jun 25, 2025

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基于长期读取的测序平台的单细胞RNA-seq分析性能的系统评估

Enze Deng1, Qingmei Shen2, Jingna Zhang3

  • 1MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China.

Journal of advanced research
|May 23, 2024
PubMed
概括

像PacBio和牛津纳米孔技术 (ONT) 这样的第三代测序 (TGS) 技术使单细胞RNA测序 (scRNA-seq) 能够分析基因拼接并识别新型异型. 在转录识别和异位基因特定表达分析方面,PacBio表现出比ONT更高的准确性.

关键词:
一个等位基因特定的基因/异形.细胞类型识别 细胞类型识别这是一种新型异构体.一个单细胞RNA测序.第三代测序系统是第三代测序系统.

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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