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Updated: Jun 21, 2026

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精确且无微流体的数字核酸量化与新月微粒模板模拟乳液

Long Chen1, Yanan Du1, Sixiang Rao1

  • 1School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.

ACS sensors
|July 12, 2025
PubMed
概括

一种新的无微流体液滴数字PCR方法使用新月微珠进行准确的核酸量化. 这项创新提高了可访问性和性能,克服了当前滴滴数字PCR系统的局限性.

关键词:
一个半月的微珠,滴滴滴滴.数字聚合酶连锁反应 (dPCR) 是一种核酸核酸是一种核酸.输入管道 输入管道 输入管道

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...