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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Single-Strand Conformation Polymorphism Mutation Analysis of the p53 Gene
1LRF Department of Haematology and Oncology Institute of Child Health, University of London, UK.
Abstract:
Single-strand conformation polymorphism (SSCP) is a rapid and convenient procedure by which differences in the base composition of short DNA strands may be detected (1, 2). A useful application of this is in the comparison of polymerase chain reaction (PCR) generated products from gene coding sequences of unknown or patient material with a known wild-type standard; where any deviation of the banding pattern produced by the former from that produced by the latter indicates a possible mutation within the sequence of that product.
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