Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

IR Spectrometers

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

Applications of IR Spectroscopy: Overview

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

IR Spectroscopy: Molecular Vibration Overview

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Unsupervised clustering FLIM-phasor from multifunctionalized nanoparticles in living cancer cells.

Methods and applications in fluorescence·2026

Same author

Resolving fluorescently labeled species using highly multiplexed spectral FLIM.

Scientific reports·2026

Same author

A novel imaging-based multi-sample viscometry application for advancing monoclonal antibody development.

Methods and applications in fluorescence·2026

Same author

Expanded applications of bioluminescence microscopy with phasor analysis.

Cell reports methods·2026

Same author

Bioluminescent Probes for Multiplexed RNA Imaging.

Journal of the American Chemical Society·2026

Same author

Unleashed condensation by recurrent mutations of an epigenetic regulator promotes cancer.

bioRxiv : the preprint server for biology·2026

Same journal

AI in Genomics: From Variant Calling to Multi-Omics Integration.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026

Same journal

Rethinking One Health: Microbial Foundations for Ecological Governance.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026

Same journal

Biobanked Liver Organoids: A Roadmap for Precision Hepatology.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026

Same journal

The Temporal Architecture of Human Cells: Organelle Clocks and Distributed Circadian Time.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026

Same journal

Opposing Activity at the Apical Surface: An Antagonistic Collaboration Between Crumbs and Myosin II Determines Organ Shape.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026

Same journal

Hidden Fungal DNA Structures May Shape Sequencing Outcomes.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 24, 2026

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

Scanning image correlation spectroscopy.

Michelle A Digman¹, Enrico Gratton

¹Laboratory for Fluorescence Dynamics, University of California, Irvine, CA, USA. mdigman@uci.edu

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology

|March 15, 2012

Summary

This summary is machine-generated.

Understanding molecular movement and interactions within cells is key to cell signaling. New fluctuation-based imaging methods map molecular behavior at the single-molecule level, revealing cellular interaction dynamics.

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Related Experiment Videos

Last Updated: May 24, 2026

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

Cellular biology
Biophysics
Molecular imaging

Background:

Molecular interactions are fundamental to cellular processes and signaling.
Understanding how molecules move and find partners within the cell is a critical, unresolved biological question.
Fluctuation-based imaging offers advanced techniques for studying these dynamics.

Purpose of the Study:

To discuss image analysis methods based on spatial and temporal correlations of fluctuations.
To explain how these methods provide insights into molecular movement and interactions within cells.
To highlight the application of these techniques for mapping cellular molecular interactions.

Main Methods:

Image analysis based on spatial and temporal correlation of fluctuations.
Raster Image Correlation Spectroscopy (RICS).
Number and Brightness (N&B) analysis.
Spatial cross-correlation analysis.

Main Results:

These methods reveal how individual molecules move and interact at the single-molecule level.
Spatio-temporal correlations of fluctuating molecules map their movement and binding in cellular compartments.
The techniques provide a cellular-level spatio-temporal map of molecular interactions.

Conclusions:

Fluctuation-based imaging methods are powerful tools for studying molecular dynamics in cells.
These techniques advance our understanding of cellular signaling by detailing molecular interactions.
Standard laser scanning microscopes can be utilized to generate comprehensive maps of molecular interactions.