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A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
Published on: October 29, 2018
Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification.
Franziska Vaagt1, Ilka Haase, Markus Fischer
1Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Hamburg, Germany.
Journal of Agricultural and Food Chemistry
|January 29, 2013
Summary
Novel loop-mediated isothermal amplification (LAMP) assays can differentiate toxic and edible mushrooms. This method, using lateral flow dipsticks, enables rapid on-site identification of poisonous species like the death cap mushroom.
Area of Science:
- Mycology
- Molecular Biology
- Food Safety
Background:
- Mushroom poisonings pose a significant public health risk, primarily caused by toxic species such as the death cap (Amanita phalloides).
- Accurate and rapid identification of toxic mushrooms is crucial to prevent accidental consumption and ensure food safety.
Purpose of the Study:
- To develop and validate novel loop-mediated isothermal amplification (LAMP) assays for differentiating closely related edible and toxic mushroom species.
- To assess the applicability of these LAMP assays for detecting toxic mushroom contamination in food products.
Main Methods:
- Development of species-specific loop-mediated isothermal amplification (LAMP) assays.
- Cross-reaction studies with various mushroom species to ensure specificity.
- Analysis of spiked mushroom samples (raw and fried) to evaluate detection limits.
- Comparison of three detection methods: agarose gel electrophoresis, real-time fluorimetric detection, and lateral flow dipstick (LFD) analysis.
Main Results:
- The developed LAMP assays successfully differentiated between closely related edible and toxic mushroom species.
- Contamination with toxic mushrooms at levels as low as 2% (w/w) was detectable in mushroom blends.
- The LAMP assay combined with LFD detection provided results in approximately 2 hours, including DNA extraction.
- The LAMP-LFD method demonstrated high sensitivity and specificity for identifying Amanita phalloides.
Conclusions:
- Loop-mediated isothermal amplification (LAMP) assays are effective tools for the rapid and accurate identification of toxic mushroom species.
- The LAMP assay coupled with lateral flow dipstick (LFD) detection offers a portable and user-friendly method for on-site detection of poisonous mushrooms.
- This technique significantly enhances food safety by enabling quick identification of toxic mushroom contamination in various food matrices.
