Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Time-of-flight fluorescence depth mapping using a spatiotemporal deep learning model.

Journal of biomedical optics·2026

Same author

Systematic validation of time-resolved diffuse optical simulators via non-contact spad-based measurements.

Physics in medicine and biology·2026

Same author

Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Accelerated SPAD-Based Diffuse Optical Tomography With Data-Driven View Optimization.

Journal of biophotonics·2026

Same author

Characterization of a fiber-coupled SPAD camera system for deep-tissue blood-flow measurement using diffuse correlation spectroscopy.

Biomedical optics express·2026

Same author

Characterization of a 3-D stacked CMOS SPAD depth sensor in 45/22-nm technology.

Optics express·2026

Same journal

NeuroFlare: An mm<sup>3</sup>-Scale Wireless Neural Interface Device with Simultaneous Neural Recording and Optical Stimulation.

IEEE journal of solid-state circuits·2026

Same journal

A Sub-mm<sup>3</sup> Wireless Neural Stimulator IC for Visual Cortical Prosthesis With Optical Power Harvesting and 7.5-kb/s Data Telemetry.

IEEE journal of solid-state circuits·2025

Same journal

A Scalable 1024-Channel Ultra-Low-Power Spike Sorting Chip with Event-Driven Detection and Spatial Clustering.

IEEE journal of solid-state circuits·2025

Same journal

Monolithic Electronic-Biophotonic System-on-Chip for Label-Free Real-Time Molecular Sensing.

IEEE journal of solid-state circuits·2025

Same journal

A Wireless, Multicolor Fluorescence Image Sensor Implant for Real-Time Monitoring in Cancer Therapy.

IEEE journal of solid-state circuits·2025

Same journal

A Nonuniform Sampling Lifetime Estimation Technique for Luminescent Oxygen Measurements for Biomedical Applications.

IEEE journal of solid-state circuits·2025

See all related articles

Search research articles

Related Experiment Video

Updated: May 8, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

A Single-Photon Avalanche Diode Array for Fluorescence Lifetime Imaging Microscopy.

David Eric Schwartz¹, Edoardo Charbon, Kenneth L Shepard

¹Bioelectronic Systems Laboratory, Department of Electrical Engineering, Columbia University, New York, NY 10027 USA (see http://www.bioee.ee.columbia.edu ).

IEEE Journal of Solid-State Circuits

|August 27, 2013

Summary

This summary is machine-generated.

This study presents an integrated single-photon avalanche diode (SPAD) imager for time-resolved fluorescence imaging. The device achieves 350 ps timing resolution, enabling precise measurement of fluorescent dye decay lifetimes.

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Related Experiment Videos

Last Updated: May 8, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Area of Science:

Photonics and Imaging
Fluorescence Spectroscopy
Integrated Circuit Design

Background:

Time-resolved fluorescence imaging requires high temporal resolution.
Existing systems often lack full integration or sufficient speed.
Advancements in single-photon avalanche diodes (SPADs) enable new imaging modalities.

Purpose of the Study:

To design and characterize a fully integrated SPAD imager for time-resolved fluorescence applications.
To demonstrate the imager's capability for precise lifetime measurements.
To offer both standard time-correlated single-photon counting (TCSPC) and gated-window detection.

Main Methods:

Development of a 64x64 array of active SPAD pixels.
Integration of an on-chip time-to-digital converter (TDC) using delay-locked loop (DLL) and calibrated interpolators.
Characterization using fluorescent dyes with varying decay lifetimes.

Main Results:

Successful design and characterization of the integrated SPAD imager.
Demonstration of time-resolved fluorescence imaging with a timing resolution of 350 ps.
Accurate measurement of fluorescent dye decay lifetimes.

Conclusions:

The integrated SPAD imager is a viable tool for advanced time-resolved fluorescence imaging.
The device's dual detection modes (TCSPC and gated-window) enhance its versatility.
This technology offers improved performance for applications requiring precise temporal information.