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Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

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Integrated Distance-Based Origami Paper Analytical Device for One-Step Visualized Analysis.

Tian Tian¹, Yuan An¹, Yiping Wu¹

¹MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University , Xiamen 361005, China.

ACS Applied Materials & Interfaces

|August 18, 2017

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This summary is machine-generated.

A novel integrated distance-based origami paper analytical device (ID-oPAD) offers simple, visual detection of targets. This low-cost platform provides quantitative results within 30 minutes, ideal for point-of-care testing in resource-limited settings.

Area of Science:

Analytical Chemistry
Biotechnology
Materials Science

Background:

Developing user-friendly diagnostic tools is crucial for point-of-care testing.
Existing methods often require complex procedures and specialized equipment.
There is a need for rapid, visual, and quantitative analytical devices, especially for resource-limited areas.

Purpose of the Study:

To develop an integrated distance-based origami paper analytical device (ID-oPAD) for simple, user-friendly, and visual detection of targets.
To achieve complete integration of target recognition, signal amplification, and visual signal output.
To provide a low-cost, disposable platform for point-of-care testing.

Main Methods:

Utilized aptamer/invertase-functionalized sepharose beads for target recognition and signal generation.

Keywords:

aptamer distance-based detection microfluidic paper-based devices point-of-care (POC)

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Employed cascaded enzymatic reactions for signal amplification.

Designed a 3D microfluidic paper-based analytical device with a distance-based readout mechanism.

Leveraged size exclusion between sepharose beads and cellulose pores for bound/free probe separation.

Main Results:

The ID-oPAD successfully integrated target recognition, signal amplification, and visual output.
A sample-in-answer-out assay was achieved within 30 minutes.
Visual and quantitative readout was obtained through a brown bar chart.
Effective separation of bound/free probes was demonstrated using size differences.

Conclusions:

The ID-oPAD is a cost-effective, disposable, and easy-to-operate analytical device.
It offers a visual and quantitative readout for target detection.
The platform is well-suited for point-of-care testing, particularly in resource-limited environments.