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Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
Published on: May 25, 2021
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Solid Phase Microextraction Techniques in Determination of Biomarkers.
Samin Hamidi1, Nastaran Alipour-Ghorbani2, Aliasghar Hamidi1,3
1a Food and Drug Safety Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Critical Reviews in Analytical Chemistry
|January 17, 2018
Summary
Accurate biomarker quantification is crucial for medical diagnostics. This review highlights advancements in solid-phase microextraction (SPME) for reliable clinical analysis of biomarkers in biological samples.
Area of Science:
- Analytical Chemistry
- Clinical Diagnostics
- Biomarker Discovery
Background:
- Biomarker detection and quantification are vital in medical research and diagnostics.
- Biomarkers exist in various forms, from gases to macromolecules, often at nanomolar concentrations.
- Complex biological matrices like plasma and urine necessitate effective sample preparation for accurate analysis.
Purpose of the Study:
- To review and discuss recent advancements in solid-phase microextraction (SPME) techniques.
- To focus on the application of SPME methods in clinical analysis.
- To emphasize the importance of reproducible and accurate data in biomarker quantification.
Main Methods:
- Review of recent progress in solid-phase microextraction (SPME) methodologies.
- Discussion of SPME applications in clinical settings.
- Evaluation of analytical method performance for biomarker quantification.
Main Results:
- Recent progress in well-established SPME methods for clinical analysis is presented.
- The importance of adequate sample preparation for nanomolar biomarker levels is discussed.
- The need for analytical methods supporting reproducible and accurate data is highlighted.
Conclusions:
- Solid-phase microextraction (SPME) is a key technique for clinical biomarker analysis.
- Advancements in SPME contribute to improved accuracy and reproducibility in diagnostics.
- Effective sample preparation is essential for reliable biomarker quantification in complex biological fluids.
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