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Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
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Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations
Tej B Shrestha1,2, Matthew T Basel3
1Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA. tbs3@vet.k-state.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 1, 2020
Summary
Luminol-based bioluminescence imaging noninvasively tracks neutrophils. This method visualizes neutrophil extravasation into inflammatory sites, particularly in mammary carcinoma models.
Area of Science:
- Biomedical imaging
- Cellular biology
- Oncology
Background:
- Luminol-based bioluminescence imaging enables noninvasive tracking of cells with oxidative activity.
- Neutrophils are key immune cells involved in inflammation and cancer progression.
Purpose of the Study:
- To describe a method for tracking neutrophil extravasation using luminol-based bioluminescence imaging.
- To specifically apply this method to visualize neutrophil infiltration in mammary carcinoma.
Main Methods:
- Intravenous or intraperitoneal administration of luminol.
- Bioluminescence imaging acquisition at 425 nm wavelength.
- Focus on monitoring neutrophil movement into inflammatory sites, including mammary tumors.
Main Results:
- Successful noninvasive visualization of neutrophil activity.
- Demonstration of tracking neutrophil extravasation into an inflammatory microenvironment.
- Application of the technique in the context of mammary carcinoma.
Conclusions:
- Luminol-based bioluminescence imaging provides a valuable tool for studying neutrophil dynamics in inflammation.
- This method facilitates the investigation of neutrophil roles in mammary carcinoma progression and anti-cancer immune responses.
