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Dose-Dependent Effect of Bacillus sp. Metabolites from Permafrost on Lymphocyte Differentiation in the Thymus
L F Kalenova1,2, S A Petrov3, A S Bazhin3,4
1Federal Research Centre Tyumen Research Centre, Siberian Division of the Russian Academy of Sciences, Tyumen, Russia. lkalenova@mail.ru.
Abstract:
We studied the effect of a single intraperitoneal injection of metabolites from Bacillus sp. M3 strain isolated from permafrost (from 5×103 to 50×103 microbial bodies) on differentiation of T cells in the thymus of F1(CBA/Black-6) mice. On day 21 after the injection of metabolites, a dose-dependent decrease in the level of CD34+CD44+ and an increase in the number of CD34+CD44-, CD25-TCR+, CD25+TCR+max, CD4+CD8-, CD4-CD8+, and CD44+TCR+ lymphocytes were observed in the thymus. The increase in thymus level of mature (CD25+TCR+max) and migration-ready (CD44+TCR+) T cells in combination with a moderate decrease in the level of T cell precursors entering the thymus from the bone marrow (CD34+CD44+) can indicate a modulating influence of Bacillus sp. metabolites on functional activity of the thymus aimed at maintenance of the T cell balance in the body.
Insights
Metabolites from permafrost bacteria Bacillus sp. M3 modulate T cell differentiation in mouse thymus. This suggests a potential role in maintaining T cell balance within the body.
Area of Science:
- Immunology
- Microbiology
- Permafrost Science
Background:
- The thymus is crucial for T cell maturation and maintaining immune homeostasis.
- Understanding microbial influences on immune cell differentiation is vital for novel therapeutic strategies.
Purpose of the Study:
- To investigate the impact of Bacillus sp. M3 metabolites on T cell differentiation in the thymus.
- To determine the dose-dependent effects of these microbial metabolites on immune cell populations.
Main Methods:
- F1(CBA/Black-6) mice received intraperitoneal injections of Bacillus sp. M3 metabolites.
- Flow cytometry was used to analyze T cell populations (CD34, CD44, CD25, TCR, CD4, CD8) in the thymus on day 21 post-injection.
Main Results:
- A dose-dependent decrease in T cell precursors (CD34+CD44+) was observed.
- Significant increases in mature T cells (CD25+TCR+max) and migration-ready T cells (CD44+TCR+) were noted.
- Changes in CD4+CD8- and CD4-CD8+ populations indicated altered T cell maturation pathways.
Conclusions:
- Bacillus sp. M3 metabolites exhibit a dose-dependent modulating effect on thymus function.
- These metabolites may influence T cell balance by promoting the maturation and migration of T cells while reducing precursor influx.
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