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NON-ENZYMATIC ELECTROCHEMICAL DETECTION OF GLUTAMATE USING TEMPLATED POLYMER-BASED TARGET RECEPTORS
Habib M N Ahmad1, Bo Si1, Gaurab Dutta1
1Department of Electrical and Computer Engineering, University of New Hampshire, USA.
International Solid-State Sensors, Actuators and Microsystems Conference : [Proceedings]. International Conference on Solid-State Sensors, Actuators, and Microsystems
|July 29, 2020
Summary
This study introduces a new electrochemical biosensor for detecting the neurotransmitter glutamate. The novel non-enzymatic sensor offers a more specific and rapid detection method for glutamate.
Area of Science:
- Electrochemistry
- Biosensing
- Materials Science
Background:
- Enzyme-based biosensors often face limitations such as limited stability, susceptibility to interference, and complex preparation.
- Current methods for detecting neurotransmitters like glutamate can be slow and lack specificity.
- There is a need for robust, specific, and rapid detection platforms for neurotransmitters.
Purpose of the Study:
- To develop a novel electrochemical biosensing platform for the detection of the neurotransmitter glutamate.
- To demonstrate a non-enzymatic approach for glutamate detection, eliminating the need for glutamate oxidase.
- To create a simpler and more robust alternative to existing enzyme-based sensors.
Main Methods:
- Utilized templated polymer-based receptors for specific molecular recognition of glutamate.
- Employed electrochemical techniques to measure conformational changes in the polymer upon glutamate binding.
- Developed a non-enzymatic sensing mechanism.
Main Results:
- Achieved specific molecular recognition of glutamate using templated polymer receptors.
- Demonstrated a measurable electrochemical signal corresponding to glutamate binding due to polymer conformation changes.
- The developed platform offers a rapid and specific response without enzymatic components.
Conclusions:
- The novel electrochemical biosensing platform provides a specific and rapid method for glutamate detection.
- The non-enzymatic approach simplifies sensor design and enhances robustness compared to enzyme-based sensors.
- This platform shows potential for monitoring various non-electroactive species and offers a promising alternative in biosensing applications.
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