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Two-Photon Microperimetry: A Media Opacity-Independent Retinal Function Assay
Ang Wei1, Urmi V Mehta1,2, Grazyna Palczewska1,3
1UCI Health Gavin Herbert Eye Institute, Department of Ophthalmology, University of California-Irvine, Irvine, CA, USA.
Translational Vision Science & Technology
|May 18, 2021
Summary
Infrared two-photon microperimetry (2PM-IR) offers more reliable retinal sensitivity measurements than visible light tests, especially with media opacities. This advanced method can detect early degenerative changes missed by conventional visual function tests.
Area of Science:
- Ophthalmology
- Vision Science
- Medical Imaging
Background:
- Conventional visual function tests can be confounded by media opacities like cataracts.
- Age-related changes in retinal sensitivity are difficult to detect with visible light testing.
Purpose of the Study:
- To compare infrared two-photon microperimetry (2PM-IR) with conventional visual function tests.
- To evaluate the impact of age and simulated media opacities on visual performance.
Main Methods:
- Two cohort studies were conducted with healthy subjects of varying ages.
- Subjects underwent testing with cone contrast threshold (CCT), conventional microperimetry, visible light microperimetry (2PM-Vis), and infrared two-photon microperimetry (2PM-IR).
- Simulated media opacities were introduced using various filters in the first cohort.
Main Results:
- 2PM-IR showed lower variability in retinal sensitivity measurements compared to visible light devices.
- Retinal sensitivity decreased with age and was affected by simulated media opacities.
- Visible light tests showed larger age-related differences inversely proportional to wavelength.
Conclusions:
- 2PM-IR effectively mitigates the masking effects of media opacities on retinal sensitivity.
- Infrared light's greater transmittance allows for reliable quantification of retinal sensitivity, aiding early detection of degenerative changes.
