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Updated: Oct 28, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
Published on: June 24, 2013
CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural
Michael A Phillips1,2,3, Maria Harkiolaki3, David Miguel Susano Pinto1
1Micron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
None:
Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.
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