A Molecular Imprinted PEDOT CMOS Chip-Based Biosensor for Carbamazepine Detection.

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Carbamazepine (CBZ) is a widely prescribed antiepileptic drug with potential environmental and health concerns.
• Accurate and sensitive detection methods for CBZ are crucial for therapeutic drug monitoring and environmental analysis.
• Existing methods for CBZ detection can be complex and time-consuming.

Purpose of the Study:

• To design, implement, and fabricate a miniaturized biosensor for the detection and quantification of carbamazepine (CBZ).
• To achieve high sensitivity and selectivity for CBZ detection using a novel approach.
• To develop a simple and easy-to-operate biosensor system.

Main Methods:

• Fabrication of a miniaturized CMOS chip biosensor coupled with a 3-electrode electrochemical cell.
• Molecular imprinting of PEDOT polymer on glassy carbon electrode (GCE) surfaces using cyclic voltammetry (CV).
• Quantification of CBZ using CV and current peak detection circuitry, validated by SEM, UV-vis spectroscopy, and electrochemical studies with a redox mediator.

Main Results:

• The biosensor demonstrated high sensitivity and selectivity towards CBZ molecules.
• Limits of detection (LOD) and quantification (LOQ) were determined to be 2.04 and 6.2 μg/mL, respectively.
• The sensor's performance was unaffected by other anticonvulsant drugs like valproic acid (VPA) and phenytoin (PHT), confirming selectivity.

Conclusions:

• A miniaturized, highly sensitive, and selective biosensor for carbamazepine detection has been successfully developed.
• The proposed biosensor offers a simple construction and user-friendly operation.
• This technology holds promise for efficient therapeutic drug monitoring and environmental analysis of CBZ.