Related Experiment Video
Updated: Aug 27, 2025

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
Analytical Principles of Cancer Next Generation Sequencing
Tatyana Gindin1, Susan J Hsiao2
1Department of Pathology, NYU Grossman School of Medicine, 240 East 38th Street, 22-66, New York, NY 10016, USA.
This article details analytical principles for cancer next-generation sequencing (NGS). It addresses challenges like low input and tumor purity, outlining lab strategies for accurate biomarker detection.
Area of Science:
- Oncology
- Genomics
- Molecular Diagnostics
Background:
- Cancer diagnostics increasingly rely on molecular profiling.
- Next-generation sequencing (NGS) is a powerful tool for identifying cancer-specific mutations.
- Challenges in analyzing cancer samples necessitate specialized approaches.
Purpose of the Study:
- To review the analytical principles of cancer next-generation sequencing (NGS).
- To highlight special considerations for processing and analyzing cancer samples.
- To discuss strategies for detecting actionable biomarkers in oncology.
Main Methods:
- Review of specimen processing techniques for cancer samples.
- Discussion of assay design considerations for cancer NGS.
- Analysis of bioinformatics approaches for cancer genomic data.
Main Results:
- Cancer samples present unique challenges: low DNA input, low tumor purity, and fixation artifacts.
- A combination of specimen handling, assay design, and bioinformatics is crucial for success.
- Successful detection of actionable biomarkers relies on optimized analytical workflows.
Conclusions:
- Effective cancer NGS testing requires tailored analytical strategies.
- Addressing sample-specific issues is key to reliable biomarker discovery.
- Optimized laboratory approaches enable robust cancer genomic analysis.
More Related Videos
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Cancer Survival Analysis
Cancers Originate from Somatic Mutations in a Single Cell
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

