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Ribozymes

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...

Catalytically Perfect Enzymes

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.

Most enzymes...

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Updated: Aug 26, 2025

Synthesis of Zeolites Using the ADOR Assembly-Disassembly-Organization-Reassembly Route

Synthesis of Zeolites Using the ADOR Assembly-Disassembly-Organization-Reassembly Route

Published on: April 3, 2016

Functional Zeolitic Imidazolate Framework for Robust l-Deoxyribozyme-Based Therapy.

Kaiyue Tan^1,2, Qingqing Zhang^1,2, Qing Wang^1,2

¹Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)

|October 10, 2022

Summary

This summary is machine-generated.

This study presents a novel left-handed deoxyribozyme (l-DNAzyme) therapy platform. It uses adenosine triphosphate (ATP) to release l-DNAzyme and supply cofactors for enhanced photodynamic therapy.

Keywords:

MicroRNAs adenosine triphosphate (ATP)left-handed deoxyribozyme (L-DNAzyme)metal-organic frameworks (MOFs)photodynamic therapy (PDT)

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Published on: March 25, 2017

Area of Science:

Biochemistry
Nanotechnology
Therapeutics

Background:

Programmable chiral biocatalysis offers precise stereospecific control but is underexplored in therapies.
Left-handed deoxyribozymes (l-DNAzymes) show nuclease resistance but face delivery and cofactor supply challenges.

Purpose of the Study:

To develop an efficient adenosine triphosphate (ATP)-stimulated system for l-DNAzyme delivery and cofactor supply.
To enhance l-DNAzyme-amplified photodynamic therapy using a self-sufficient therapeutic platform.

Main Methods:

Integration of l-histidine (l-His) into ZIF-8 nanoparticles (l-His-ZIF-8) for controlled release.
Utilizing endogenous ATP to trigger the disassembly of l-His-ZIF-8, releasing l-DNAzyme and l-His cofactors.
Employing microRNA-21 to catalyze l-DNAzyme generation for photosensitizer activation.

Main Results:

Demonstrated efficient ATP-stimulated disassembly of l-His-ZIF-8.
Successfully released l-DNAzyme probes and supplied l-His cofactors.
Achieved amplified photodynamic therapy through microRNA-21-catalyzed l-DNAzyme activation.

Conclusions:

The developed l-therapeutic platform enables intelligent release of l-DNAzyme and cofactor supply.
This strategy enhances photodynamic therapy efficacy through sustained l-DNAzyme amplification.
Presents a promising approach for precise diagnosis and customized gene silencing therapies.