Related Experiment Video
Updated: Aug 19, 2025
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
34.2K
Prospective Advances in Genome Editing Investigation
1Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Catania, Italy. gaetano.isola@unict.it.
Advances in Experimental Medicine and Biology
|December 1, 2022
Summary
Genome editing technologies, including CRISPR/Cas9, offer adaptable tools for genetic disorder treatment. These advancements enable precise gene expression control for conditions like cardiovascular diseases and cancer.
Area of Science:
- Genomic editing technologies
- Molecular biology
- Biomedical applications
Background:
- Genome editing has advanced significantly since 2010.
- CRISPR/Cas9 is a key genome-editing instrument derived from microbial defense systems.
- CRISPR/Cas9 offers ease of use, effectiveness, and adaptability in biomedical research.
Purpose of the Study:
- To discuss conceived genome editing technologies.
- To explore new perspectives for treating genetic disorders.
- To highlight the application of gene editing in modulating epigenetics for disease treatment.
Main Methods:
- Utilizing clustered regularly interspaced short palindromic repeat (CRISPR) DNA sequences/CRISPR-associated (Cas) type-9 method.
- Employing genetic manipulation tools derived from microbial host response systems.
- Applying reversible and modulating control of gene expression epigenetics.
Main Results:
- CRISPR/Cas9 has become an important advancement in genome editing.
- The technology shows great adaptability to different biomedical areas.
- Potential applications exist for treating cardiovascular diseases, metabolic diseases, inflammatory diseases, and tumors.
Conclusions:
- Genome editing technologies, particularly CRISPR/Cas9, represent a significant leap in genetic manipulation.
- These techniques offer promising avenues for treating a range of genetic disorders.
- Future applications may involve precise epigenetic control for therapeutic interventions.
Related Concept Videos
01:28CRISPR/Cas9 Genome Editing
132
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
132
01:59CRISPR
52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
00:49What is Genetic Engineering?
74.6K
Overview
74.6K
02:53CRISPR and crRNAs
17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
02:31Homologous Recombination
50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K
02:23RNA Editing
9.1K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K