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Related Experiment Video

Updated: Aug 15, 2025

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

Refining Enteric Neural Circuitry by Quantitative Morphology and Function in Mice.

Marthe J Howard¹

¹University of Toledo, College of Medicine and Life Sciences, Toledo, OH, USA. Marthe.Howard@utoledo.edu.

Advances in Experimental Medicine and Biology

|December 31, 2022

Summary

This summary is machine-generated.

This study quantifies the mouse colon's enteric nervous system (ENS), revealing how structural variations impact neural circuit dynamics. This provides a better model for understanding human gut motility disorders.

Keywords:

ENS Enteric morphology Neural circuits Optogenetics Synaptic function

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Area of Science:

Neuroscience
Gastroenterology
Comparative Anatomy

Background:

Mouse models are crucial for studying the enteric nervous system (ENS) and its role in gut motility disorders.
A lack of comprehensive quantitative data on the murine colonic ENS hinders its effective use as a model for human conditions.

Purpose of the Study:

To provide a quantitative description of the mouse colon's ENS architecture, connectivity, and function.
To assess the utility of the mouse colon as a surrogate for studying the human and other mammalian ENS.

Main Methods:

Quantitative confocal microscopy and 3D image reconstruction.
GCaMP imaging combined with pharmacological interventions.
Analysis of neural circuit dynamics and functional connectivity.

Main Results:

Demonstrated regional structural variations and variable neuronal distribution within the mouse colon.
Established that these architectural features define neural circuit dynamics and functional connectivity.
Showcased how these factors are responsible for colonic motor patterns and regional functional differences.

Conclusions:

The quantitative data advances the utility of multispecies and gut region-specific data for ENS research.
Highlights the importance of structural and functional characterization for accurate modeling of gut disorders.
Supports the use of the mouse colon as a relevant model for human ENS research, with considerations for regional specificity.