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Selective Aptamer Modification of Au Surfaces in a Microelectrode Sensor Array for Simultaneous Detection of Multiple
Debashis Sen1,2, Robert A Lazenby1
1Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306-4390, United States.
Analytical Chemistry
|April 18, 2023
Summary
Researchers developed a new method for modifying microelectrodes with specific aptamers for simultaneous detection. This technique enables multiplexed sensing on closely spaced electrodes, advancing electrochemical aptamer-based (E-AB) biosensors.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Nanotechnology
Background:
- Electrochemical aptamer-based (E-AB) biosensors utilize aptamers for analyte detection.
- Simultaneous detection of multiple analytes requires selective modification of individual electrodes.
- Existing methods are unsuitable for closely spaced microelectrode arrays.
Purpose of the Study:
- To develop a selective modification method for microelectrode surfaces in E-AB biosensors.
- To enable simultaneous multianalyte detection using aptamers on microelectrode arrays.
Main Methods:
- Selective surface modification of microelectrodes using thiolated aptamers.
- Controlled potential-induced electrodesorption of thiol monolayers to expose gold surfaces.
- Successive addition of different thiolated aptamers for multiplexed aptamer probe immobilization.
- Methylene blue termination of aptamers for monitoring probe density and sensor selectivity via redox currents.
Main Results:
- Demonstrated selective modification of closely spaced microelectrodes with different aptamers.
- Successfully employed methylene blue-terminated aptamers to monitor probe packing density and sensor selectivity.
- Validated the microscale E-AB sensor multianalyte detection method for adenosine triphosphate, dopamine, and serotonin.
Conclusions:
- Developed a novel technique for selective aptamer functionalization of microelectrode arrays.
- This method facilitates simultaneous multianalyte detection in E-AB biosensors.
- The approach is applicable for localized, simultaneous sensing using electrode arrays.
