Related Experiment Video
Updated: Jun 14, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
How Fast is Your Detector? The Effect of Temporal Response on Image Quality
Tiarnan Mullarkey1,2, Matthew Geever1, Jonathan J P Peters1
1School of Physics, Trinity College Dublin, College Green, Dublin D02 PN40, Ireland.
Abstract:
With increasing interest in high-speed imaging, there should be an increased interest in the response times of our scanning transmission electron microscope detectors. Previous works have highlighted and contrasted the performance of various detectors for quantitative compositional or structural studies, but here, we shift the focus to detector temporal response, and the effect this has on captured images. The rise and decay times of eight detectors' single-electron response are reported, as well as measurements of their flatness, roundness, smoothness, and ellipticity. We develop and apply a methodology for incorporating the temporal detector response into simulations, showing that a loss of resolution is apparent in both the images and their Fourier transforms. We conclude that the solid-state detector outperforms the photomultiplier tube-based detectors in all areas bar a slightly less elliptical central hole and is likely the best detector to use for the majority of applications. However, using the tools introduced here, we encourage users to effectively evaluate which detector is most suitable for their experimental needs.
Related Concept Videos
Difference from Background: Limit of Detection
The LOD indicates the presence or absence...
Determination of Crystal Structures

