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Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

231
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
231
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

378
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
378

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Related Experiment Video

Updated: Jul 13, 2025

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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Sample Preparation in Microbial Metabolomics: Advances and Challenges.

Heiter V M Boness1, Hanna C de Sá1, Emile K P Dos Santos1

  • 1Department of Analytical Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, BA, Brazil.

Advances in Experimental Medicine and Biology
|October 16, 2023
PubMed
Summary
This summary is machine-generated.

Microbial metabolomics reveals microorganism physiology. Proper sample preparation is crucial for reliable results, impacting data quality in this field.

Keywords:
Cellular cultivationExtracellular metabolomeIntracellular metabolomeMetabolic quenchingMetabolite extractionMicrobial metabolomicsSample preparation

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Area of Science:

  • Microbiology
  • Metabolomics
  • Biochemistry

Background:

  • Microbial metabolomics is vital for understanding microorganism physiology.
  • Metabolite concentrations and organism variability necessitate careful sample preparation.
  • Sample preparation directly influences the quality and reliability of metabolomic data.

Purpose of the Study:

  • To provide a comprehensive overview of microbial metabolomics sample preparation.
  • To highlight critical pre-analytical factors in the metabolomic workflow.
  • To discuss methods for obtaining both intracellular and extracellular metabolites.

Main Methods:

  • Overview of sample preparation workflow for microbial metabolomics.
  • Discussion of pre-analytical factors: cultivation, harvesting, metabolic quenching, and extraction.
  • Introduction to advanced automated sample preparation systems.

Main Results:

  • Sample preparation procedures are contingent on microorganism type, metabolomics approach (targeted/untargeted), and analytical platform.
  • Effective methods for isolating intracellular and extracellular metabolites are presented.
  • Advanced automated techniques offer enhanced efficiency in sample preparation.

Conclusions:

  • Optimized sample preparation is essential for accurate microbial metabolomics.
  • The choice of method impacts the scope and reliability of metabolite analysis.
  • Automated systems represent a future direction for microbial metabolomics sample preparation.