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Published on: June 27, 2022
A cysteine-activated fluorescent H2S donor for visualizing H2S release and alleviating cellular inflammation
Nianwei Wang1, Xuan Wang1, Jicheng Wang1
1Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, P. R. China. lidahong0203@163.com.
Abstract:
We developed Bcy-NCS, a Cys-specific fluorescent H2S donor with a self-immolative spacer, overcoming the issue of multiple fluorophore release seen in traditional ICT-based donors. Bcy-NCS selectively responds to Cys, releases H2S, and enables fluorescence-based monitoring. It also reduces LPS-induced inflammation in RAW 264.7 cells, indicating potential for inflammation treatment.
