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Acoustic Bioprinting: Liquid-Bridge-Based Efficient Single-Cell Encapsulation Using Gigahertz Bulk Acoustic Waves
Yangchao Zhou1,2, Meihang He2, Xuexin Duan2
1School of Aeronautical Engineering, Taizhou University, Taizhou 318000, Zhejiang, China.
Analytical Chemistry
|March 2, 2026
Summary
This study introduces an acoustic bioprinting method for highly efficient single cell encapsulation. The novel liquid bridge transfer achieves nearly 100% efficiency and improved resolution for biological applications.
Area of Science:
- Biotechnology
- Bioengineering
- Cell Biology
Background:
- Current single cell encapsulation methods face limitations in spatial resolution, operational complexity, and biocompatibility.
- Low encapsulation efficiency leads to significant biological sample waste.
Purpose of the Study:
- To develop an acoustic-based bioprinting method for enhanced single cell encapsulation.
- To overcome the limitations of existing cell encapsulation techniques.
Main Methods:
- Utilized an acoustic-based bioprinting approach with a liquid bridge for cell transfer.
- Integrated an optical feedback system for precise control.
- Leveraged ultrahigh resonant frequency and a small device footprint.
Main Results:
- Achieved nearly 100% single cell encapsulation efficiency.
- Improved encapsulation resolution to below 100 μm.
- Demonstrated combined encapsulation of single cells and particles.
Conclusions:
- The proposed liquid-bridge bioprinting technology offers high efficiency and resolution for single cell encapsulation.
- The method shows versatility for various micro-objects and good biocompatibility.
- Potential applications include cell assembly, chemical analysis, and biofabrication.
