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Epithelial cells retain junctions during mitosis

J Baker1, D Garrod

  • 1Cancer Research Campaign, Medical Oncology Unit, University of Southampton, Southampton General Hospital, UK.

Journal of Cell Science
|February 1, 1993
PubMed
Summary
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Epithelial cells maintain cell-cell junctions during mitosis, unlike their reduced cell-substratum adhesion. This retention of intercellular junctions is crucial for tissue integrity during cell division.

Area of Science:

  • Cell Biology
  • Histology
  • Tissue Engineering

Background:

  • Cell-substratum adhesion decreases during mitosis.
  • The behavior of cell-cell adhesion during mitosis is less understood.
  • Epithelial cells utilize intercellular junctions like desmosomes, tight junctions, and zonulae adherentes.

Purpose of the Study:

  • To investigate whether epithelial cells disassemble intercellular junctions during cell division.
  • To determine if intercellular junctions persist in both cultured and in-situ dividing epithelial cells.

Main Methods:

  • Fluorescent staining of cultured epithelial cells for desmosomes, tight junctions, and zonulae adherentes.
  • Light and electron microscopy to examine junctional structures during mitosis.
  • Electron microscopy analysis of dividing human colonic mucosal crypt cells and basal keratinocytes.

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Main Results:

  • Cultured epithelial cells retained all examined intercellular junctions (desmosomes, tight junctions, zonulae adherentes) throughout mitosis.
  • No evidence of junction internalization was observed in dividing cultured cells.
  • Dividing epithelial cells in human colonic mucosa and basal keratinocytes also retained their intercellular junctions.
  • Dividing basal keratinocytes maintained hemidesmosomal contact with the basement membrane.

Conclusions:

  • Intercellular junctions are retained by epithelial cells during cell division, both in vitro and in vivo.
  • The persistence of cell-cell junctions during mitosis is vital for maintaining tissue integrity and organization.