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Updated: Jul 19, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
A posteriori quasi-sectioning of the three-dimensional reconstructions of scanning holographic microscopy
1Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA. gindebet@vt.edu
Abstract:
A method to obtain the axial locations of objects reconstructed by scanning holographic microscopy with submicrometer accuracy is presented and demonstrated. The method combines the holographic advantage of capturing three-dimensional (3D) information in a single two-dimensional scan, with the possibility of optically sectioning the 3D holographic reconstruction a posteriori. The method is demonstrated experimentally for pointlike features (fluorescent beads), and the limitation of the method for features of arbitrary size and shape is discussed.
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