Multienzyme System in Amorphous Metal-Organic Frameworks for Intracellular Lactate Detection

Area of Science:

• Biochemistry
• Nanotechnology
• Cell Biology

Background:

• Lactate is a key metabolite in glycolysis, and its levels are linked to various diseases.
• Accurate intracellular lactate detection is crucial for understanding cellular metabolism and disease states.

Purpose of the Study:

• To develop a novel multienzyme system for sensitive and selective intracellular lactate detection.
• To utilize amorphous metal-organic frameworks (aMOFs) as a platform for constructing artificial organelles.

Main Methods:

• Coencapsulation of lactate oxidase (LOx) and horseradish peroxidase (HRP) within aMOFs.
• Delivery of the aMOF-based multienzyme system into living cells.
• Utilizing proximity-enhanced enzyme activity for signal amplification.

Main Results:

• The engineered multienzyme system demonstrated high activity and selectivity for intracellular lactate.
• The system improved the signal-to-noise ratio for lactate detection by approximately 650-fold.
• Experimental and simulation data confirmed that enzyme proximity within aMOFs enhanced detection efficiency.

Conclusions:

• The construction of multienzyme systems within aMOFs provides a promising strategy for intracellular metabolite detection.
• This approach offers a novel method for creating artificial organelles for enhanced biological sensing.
• The developed system holds potential for advancing diagnostics and research in metabolic diseases.